Journal: Dentistry Journal

Article Title: Study on the Influence of Hydroxyapatite on Human Cell Viability and Adhesion in Chemical Antibacterial Silver Coatings

doi: 10.3390/dj14040202

Figure Lengend Snippet: Viability of human fetal osteoblasts (hFOB cell line) after 8 days of incubation on the surface of uncoated and coated TiZr samples. The results are calculated as mean ± standard deviation from three independent experiments and are presented relative to the control—the plastic surface of the plate with 96 wells; * p < 0.05 and ** p < 0.01 compared to control.

Article Snippet: Human osteoblasts (hFOB 1.19-CRL-11372 cell line, acquired from ATCC, Manassas, VA, USA) were used for in vitro testing of cell viability and adhesion.

Techniques: Incubation, Standard Deviation, Control

Journal: Dentistry Journal

Article Title: Study on the Influence of Hydroxyapatite on Human Cell Viability and Adhesion in Chemical Antibacterial Silver Coatings

doi: 10.3390/dj14040202

Figure Lengend Snippet: Fluorescent staining of live cells (green labeling with calceine solution) and dead cells (red labeling with propidium iodide) after exposure of human osteoblasts to TiZr surfaces coated with Ag and HAP. All images were obtained with the 20× objective, scale bar: 50 μm.

Article Snippet: Human osteoblasts (hFOB 1.19-CRL-11372 cell line, acquired from ATCC, Manassas, VA, USA) were used for in vitro testing of cell viability and adhesion.

Techniques: Staining, Labeling

Journal: Dentistry Journal

Article Title: Study on the Influence of Hydroxyapatite on Human Cell Viability and Adhesion in Chemical Antibacterial Silver Coatings

doi: 10.3390/dj14040202

Figure Lengend Snippet: Fluorescent labeling of actin filaments in human osteoblasts after 8 days of incubation with Ag- and HAP-coated samples (green: F-actin labeled with phalloidine-fluorescein isothiocyanate; blue: nuclei labeled with 4′,6-diamidino-2-phenylindole; scale bar: 100 μm).

Article Snippet: Human osteoblasts (hFOB 1.19-CRL-11372 cell line, acquired from ATCC, Manassas, VA, USA) were used for in vitro testing of cell viability and adhesion.

Techniques: Labeling, Incubation

Journal: Journal of the American Ceramic Society. American Ceramic Society

Article Title: ZnO-Hydroxyapatite-Coated Ti-6Al-4V With Curcumin and Ginger Extract for Load-Bearing Implants

doi: 10.1111/jace.70532

Figure Lengend Snippet: Assessment of in vitro properties of the drug-loaded coated implants (a) MTT assay indicates that no compositions are cytotoxic. On Day 3, the tested compositions show similar cell viability. Significantly higher viability of cells is noticed on the treatment on Days 7 and 11 as compared to the control. The Zn-HAP-Cur sample shows ~1.3 times enhanced cell viability, and ~1.5 times enhancement in cell viability is observed for the Zn-HAP-Cur-Gin composition. A p value of <0.001 is denoted by ***. The FESEM images on Day 11 show good cellular attachment covered within the apatite layer. Arrows in the FESEM images denote osteoblast cells. (b) MTT assay after osteosarcoma cell–implant interactions indicates that the cell viability significantly decreases due to curcumin and ginger extract. On Day 3, the Zn-HAP-Cur composition shows a ~1.2-fold reduction in cell viability compared to the control. On Days 7 and 11, further reduction in cellular viability is noticed, with a ~3.7 times reduction on Day 7 and a ~7.3 times reduction on Day 11. The Zn-HAP-Cur-Gin composition shows ~11 times decrease in osteosarcoma viability on Day 11. The FESEM images show the presence of cells and are marked with arrows, (c) the agar plate photographs after implant– S. aureus interaction for 36 h show a significant reduction of bacteria on the treatment sample as compared to the control, (d) quantification of bacterial cell viability indicates that The Zn-HAP-Cur-Gin composition shows ~92% antibacterial efficacy *** denotes a p value of < 0.001, (e) schematic of the osteoblast proliferation, osteosarcoma inhibition, and bacterial killing due to the actions of Zn 2+ , curcumin, and ginger extracts.

Article Snippet: Human fetal osteoblast cells (hFOB 1.19, obtained from ATCC, Manassas, VA, USA) were cultured on the fabricated samples for 3, 7, and 11 days [ ].

Techniques: In Vitro, MTT Assay, Control, Cell Attachment Assay, Bacteria, Inhibition